Hygromycin B 纯度:>98.00% 5g

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页酶、辅酶及抑制剂MCE>

图片仅供参考，请以实物为准

Hygromycin B 纯度:>98.00% 5g

市场价：: 3300.00

促销价：: ¥3300.00

货号：HY-B0490

品牌：MCE

配送至：

北京朝阳

支付：账期货到付款

数量：

支 (预计4-5工作日到货(以MCE官网货期为准))

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Hygromycin B是对原核和真核细胞有活性的氨基糖苷类抗生素。

Description

Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells.

IC₅₀ & Target

Target：Antibacterial; Antifungal^[1]

In Vitro

Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus^[1]. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site^[2].

In Vivo

Hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading in vivo^[3]. Constitutive expression of the bacterial hyg^R gene in transgenic mice in vivo confers resistance to hygromycin B^[4].

Solvent & Solubility

In Vitro:

H₂O : ≥ 30 mg/mL (56.87 mM)

* "≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8957 mL	9.4783 mL	18.9566 mL
5 mM	0.3791 mL	1.8957 mL	3.7913 mL
10 mM	0.1896 mL	0.9478 mL	1.8957 mL

* Please refer to the solubility information to select the appropriate solvent.

References

[1]. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98.

[2]. González A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec 21;521(2):459-69.

[3]. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502.

Animal Administration ^[4]	Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hyg^R are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days^[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98. [2]. González A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec 21;521(2):459-69. [3]. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502.

Animal Administration
^[4]

Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hyg^R are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98.

[2]. González A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec 21;521(2):459-69.

[3]. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502.

Molecular Weight

527.52

Formula

C₂₀H₃₇N₃O₁₃

CAS No.

31282-04-9

SMILES

O[C@H]([C@H]1O)C2(O[C@@]([C@@H](N)CO)([H])[C@@H]1O)O[C@@]([C@@H](O[C@@H]3CO)O[C@@]([C@H]([C@H](N)C4)O)([H])[C@@H]([C@H]4NC)O)([H])[C@@]([C@H]3O)([H])O2

Storage