G-418 disulfate 是一种氨基糖苷类抗生素,与 gentamicin B1 的结构相似,在原核和真核细胞的蛋白质合成过程中,能够阻止蛋白质的延伸阶段,从而抑制蛋白质的合成。
Description | G-418 (disulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, which blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. | ||||||||||||||||
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In Vitro | G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/mL. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells[1]. In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks[2]. | ||||||||||||||||
In Vivo | G418 (40 and 80 mg/kg) for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice[3]. | ||||||||||||||||
Solvent & Solubility | In Vitro: H2O : ≥ 30 mg/mL (43.31 mM) * "≥" means soluble, but saturation unknown. Preparing Stock Solutions
* Please refer to the solubility information to select the appropriate solvent. | ||||||||||||||||
References | |
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