Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents. Provided as 1 ml vial containing the following components: Imidazole (200 mM), Sodium Fluoride (100 mM), Sodium Molybdate (115 mM), Sodium Orthovanadate (100 mM), and Sodium Tartrate Dihydrate (400 mM).

Phosphatase Inhibitor Cocktail II has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in ddH2O.

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The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Ingredient	M.W.	100x Conc.	Target
Sodium Fluoride	42.0	100 mM	Acid phosphatases
Sodium Orthovanadate	183.9	100 mM	Alkaline phosphatases, PTPs, ATPases
Sodium Tartrate Dihydrate	230.1	400 mM	Acid phosphatases
Sodium Molybdate	205.9	115 mM	Acid and phosphoprotein phosphatases
Imidazole	68.1	200 mM	Alkaline phosphatases