Recombinant Human Actin, cytoplasmic 1/ACTB 10ug

Known as	Actin Cytoplasmic 1; Beta-Actin; ACTB
Derived from	E.coli
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Formulation	Supplied as a 0.2 μm filtered solution of 10mM TrisHCl, 0.1% TritonX-100, 2mM DTT, pH 8.0.
Shipping	The product is shipped on dry ice/ice packs.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months
Endotoxin	Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Background	Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Known as

Actin Cytoplasmic 1; Beta-Actin; ACTB

Derived from

E.coli

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Formulation

Supplied as a 0.2 μm filtered solution of 10mM TrisHCl, 0.1% TritonX-100, 2mM DTT, pH 8.0.

Shipping

The product is shipped on dry ice/ice packs.

Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months

Endotoxin

Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

Background

Actins are ubiquitous globular and highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. ACTB is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Recombinant Human Actin, cytoplasmic 1/ACTB 10ug

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