Recombinant Human PDGF-BB 50ug

Known as	Platelet-Derived Growth Factor Subunit B; PDGF Subunit B; PDGF-2; Platelet-Derived GrowthFactor B Chain; Platelet-Derived Growth Factor Beta Polypeptide; Proto-Oncogene c-Sis;Becaplermin; PDGFB; PDGF2; SIS Becaplermin; PDGFB; PDGF2; SIS
Derived from	E.coli
Purity	Measured by its ability to binding PDGFR2B used funtional ELISA.The ED50 for this binding effect is 5.04ug/ml when PDGFB 1ug/ml in a solid phases.Greater than 95% as determined by reducing SDS-PAGE.
Formulation	Lyophilized from a 0.2 μm filtered solution of 4mM HCl.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 15 months
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in13X PBS.Please aliquot the reconstituted solution to minimize freeze-t cycles.
Endotoxin	Less than 0.1 ng/ug (1 IEU/ug).
Amino Acid Sequence	MSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIF KKATVTLEDHLACKCETVAAARPVT
Background	Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Plateletderived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGFAB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

Known as

Platelet-Derived Growth Factor Subunit B; PDGF Subunit B; PDGF-2; Platelet-Derived GrowthFactor B Chain; Platelet-Derived Growth Factor Beta Polypeptide; Proto-Oncogene c-Sis;Becaplermin; PDGFB; PDGF2; SIS Becaplermin; PDGFB; PDGF2; SIS

Derived from

E.coli

Purity

Measured by its ability to binding PDGFR2B used funtional ELISA.The ED50 for this binding effect is 5.04ug/ml when PDGFB 1ug/ml in a solid phases.Greater than 95% as determined by reducing SDS-PAGE.

Formulation

Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 15 months

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in13X PBS.Please aliquot the reconstituted solution to minimize freeze-t cycles.

Endotoxin

Less than 0.1 ng/ug (1 IEU/ug).

Amino Acid Sequence

MSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIF KKATVTLEDHLACKCETVAAARPVT

Background

Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Plateletderived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGFAB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Recombinant Human PDGF-BB 50ug

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