Recombinant Human EGF 500ug

Known as	Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Derived from	E.coli
Purity	Measured by the dose-dependent proliferation of murine BALB/c 3T3 cells.ED50 is less than 2 ng/ml.Greater than 95% as determined by reducing SDS-PAGE.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, pH 7.0.
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Storage	Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 17 months
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in15X PBS.Please aliquot the reconstituted solution to minimize freeze-t cycles.
Endotoxin	Less than 0.1 ng/ug (1 IEU/ug).
Amino Acid Sequence	NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Background	Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

Known as

Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone

Derived from

E.coli

Purity

Measured by the dose-dependent proliferation of murine BALB/c 3T3 cells.ED50 is less than 2 ng/ml.Greater than 95% as determined by reducing SDS-PAGE.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, pH 7.0.

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 17 months

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in15X PBS.Please aliquot the reconstituted solution to minimize freeze-t cycles.

Endotoxin

Less than 0.1 ng/ug (1 IEU/ug).

Amino Acid Sequence

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Background

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Recombinant Human EGF 500ug

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