描述
Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of
Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I
modifications for a range of cell culture applications.
This Opti-MEM® I is modified as follows: The complete formulation is confidential. For more information, please contact
Technical Services.
Using Opti-MEM® I Medium Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO
2 environment to maintain physiological pH.
cGMP manufacturing and quality system Opti-MEM® I is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Scotland facility (51985-042). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
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