描述

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Life Technologies offers a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO ₂ incubator, or with Hank’s salts for use without CO ₂. Gibco® MEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical Gibco® MEM product made in our Grand Island facility (42360-099). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 and ISO 9001 standards.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO ₂ environment to maintain physiological pH.

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

规格

Glutamine:	GlutaMAX™-I
Phenol Red Indicator:	Phenol Red
Form:	Liquid
HEPES Buffer:	HEPES
Sodium Pyruvate Additive:	No Sodium Pyruvate
Classification:	Animal Origin-Free
Concentrated:	1 X
Culture Environment:	CO2
Osmolality:	280 - 320 mOsm/kg
Product Line:	Gibco®, GlutaMAX™
Product Size:	10 x 500 mL
Serum Supplementation:	Standard Serum Supplementation
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Supplementation Required:	Serum
Volume (Metric):	500 ml
pH Range:	7.1 - 7.3
Green Features:	Sustainable packaging

内容及储存