描述
The Bac-to-Bac® HT Vector is designed for use as part of the Bac-to-Bac® Baculovirus Expression System (Cat. No. 10359-016) for the expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli. The pFastBac™ HT vector offers the following features:
• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6xHis tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of the histidine tag following protein purification 规格
| Cleavage: | TEV Protease Recognition Site |
| Cloning Method: | Restriction Enzyme ⁄ MCS |
| Product Line: | Bac-to-Bac™ |
| Product Size: | 1 kit |
| Promoter: | Polyhedrin |
| Protein Tag or Fusion: | His Tag (6x) |
| Vector Type: | pFastBac |
内容及储存
The Bac-to-Bac® HT Vector Kit includes 10 µg of pFastBac™ HT vector and pFastBac HT-CAT control vector.
Store the vectors at 2–8°C. All components are guaranteed stable for 6 months when properly stored.
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