描述
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the
media selector tool.
This DMEM / F-12 is modified as follows: | With | Without |
| • GlutaMAX™ | • HEPES |
The complete
formulation is available.
Using DMEM/F-12 DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with
GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10%
Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO
2 environment to maintain physiological pH.
cGMP manufacturing and quality system DMEM/F-12 is manufactured at a cGMP-compliant facility located in Paisley, Scotland. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Grand Island facility (10565-018). This facility is also registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
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