描述
Expi293F™ human cells are derived from the 293 cell line, and are a core component of the Expi293™ Expression System. They are maintained in suspension culture and will grow to high density in Expi293™ Expression Medium. Expi293F™ cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.
• Suspension culture cells pre-adapted to Expi293™ Expression Medium
• Demonstrated higher protein expression levels than other 293 cells adapted to Expi293™ Expression Medium
• Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells
• Consistent transient expression performance of Expi293F™ cells for over 40 passages beyond thaw
Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 10 7 viable cells/ml in Expi293™ Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293™ Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.
Cells are typically grown in flasks on a shaker platform in a humidified, CO 2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression. 规格
Species: | Human |
Cell Line: | Expi293F™ |
Cell Type: | Kidney (Embryonic) |
Culture Type: | Suspension Cell Culture |
Form: | Cryopreserved |
Media Adaptation: | Expi293™ Expression Medium |
Number of Cells: | 1 x 107 |
Parental Cells: | FreeStyle™ 293-F Cells |
Product Line: | Expi293™ |
Product Size: | 6 x 1 mL |
Quantity: | 6 vial(s) |
System: | Expi293™ Expression System |
内容及储存
1 x 10 7 cells⁄mL, store in liquid nitrogen