描述
The ExpiSf Expression System is a complete chemically-defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow.
The ExpiSf Expression System offers:
• A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
• Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
• Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• A fast, simplified workflow that allows you to obtain protein in as little as 6–10 days; half the time of traditional protocols
• Scalable expression that can be applied to cultures from 24-deep well plates to >3 L shake flasks
The components includes in the ExpiSf Expression System Starter Kit are: 2 vials of frozen ExpiSf9 Cells, 1 liter of ExpiSf CD Medium, ExpiSf Enhancer sufficient for 1 liter of culture, and 1 mL ExpiFectamine Sf Transfection Reagent. All components of the ExpiSf Expression System are also available for purchase separately.
规格
Cell Line: | Sf9, SFM Adapted |
Delivery Method: | Baculovirus |
Product Line: | ExpiSf™ |
Product Size: | 1 kit |
Promoter: | Polyhedrin |
Shipping Condition: | Approved for shipment at Room Temperature or on Wet or Dry Ice |
内容及储存
• 1000 mL bottle ExpiSf CD Medium, store in the dark at 2–8°C
• 4 x 1 mL ExpiSf Enhancer, 2–8°C
• 1 mL ExpiFectamine Sf Transfection Reagent, 2–8°C
• 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen