描述

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g / L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. Gibco® DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM product made in our Grand Island facility (10564-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 and ISO 9001 standards.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g / L) and therefore requires a 5-10% CO ₂ environment to maintain physiological pH.

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

规格

Glutamine:	GlutaMAX™-I
Phenol Red Indicator:	Phenol Red
Form:	Liquid
Glucose:	High Glucose
Serum Supplementation:	Standard Serum Supplementation
HEPES Buffer:	HEPES
Sodium Pyruvate Additive:	No Sodium Pyruvate
Classification:	Animal Origin-Free
Concentrated:	1 X
Culture Environment:	CO2
Osmolality:	270 - 310 mOsm/kg
Product Line:	Gibco®, GlutaMAX™
Product Size:	10 x 500 mL
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Supplementation Required:	Serum
Volume (Metric):	500 ml
pH Range:	7.1 - 7.3
Green Features:	Sustainable packaging

内容及储存