描述
The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium
• Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
• Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
• Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
• Provides robust and reproducible transfection results, giving users greater confidence in their results
• Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields
The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 16 to 18 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX. 规格
Cell Type: | Established Cell Lines |
Sample Type (Specific): | Plasmid DNA |
Classification: | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
Transfection Technique: | Lipid-Based Transfection |
Serum Compatible: | No |
Cell Line: | Expi293F™ |
Product Line: | ExpiFectamine™ |
Product Size: | For 10 L of culture |
Purity or Quality Grade: | Cell Culture Grade |
内容及储存
27 mL ExpiFectamine™ 293 Reagent
50 mL ExpiFectamine™ 293 Transfection Enhancer 1
500 mL ExpiFectamine™ 293 Transfection Enhancer 2
Sufficient for transfection of 10 L of culture
Store at +2°C to +8°C