StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic collagenolytic enzymes. Accutase® does not contain mammalian bacterial derived products is useful for the routine detachment of cells from standard tissue culture plasticware adhesion coated plasticware, including Geltrex™, CELLStart™ polymers. Accutase® performs exceptionally well in detaching primary stem cells maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), flow cytometry. Cell lines tested for Accutase® application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, established cell lines such as adherent CHO BHK cells 293 cells. A complete list of published references is available on our website. Accutase® is a trademark of ICT, Inc.