描述
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. Life Technologies offers a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the
media selector tool.
This MEM α is modified as follows:
With |
• Ribonucleosides |
• Deoxyribonucleosides |
• GlutaMAX™ |
The complete
formulation is available.
Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B
12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Gibco® MEM α with
GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.
Product Intended Use For
in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System Gibco® MEM α is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical Gibco® MEM α product made in our Grand Island facility (32571-101). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 and ISO 9001 standards.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10%
Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO
2 environment to maintain physiological pH.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)