描述
ExpiSf CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (
Spodoptera frugiperda) insect cells.
ExpiSf CD Medium:
• Delivers high reproducibility and lot-to-lot reliability
• Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms
• Supports growth of suspension Sf9 cultures to densities of approximately 2 x 10
7 cells/mL, with >90% cell viability
• Supports high-density infection of suspension Sf9 cells with recombinant baculovirus
• Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >2 L in shaker flasks
• Is a ready-to-use formulation, with no supplementation required
ExpiSf CD Medium is a core component of the
ExpiSf Expression System. For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the
ExpiSf Protein Production Kit. Sf21 cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn’t guaranteed. ExpiSf CD Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO
2 cell culture incubator.
cGMP manufacturing ExpiSf CD Medium is manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP).
Dry media formulations For added flexibility, ExpiSf CD Medium can be made in AGT dry format at large scale. For custom media requests, please contact
Gibco Custom Media Services.
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