Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, primary fibroblasts. We offer a variety of Opti-MEM® I modifications for a range of cell culture applications.

The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM® I Medium
Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), therefore requires a 5–10% CO ₂ environment to maintain physiological pH.

cGMP manufacturing quality system
Opti-MEM® I is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Grand Island facility (31985-070). This facility is also registered with the FDA as a medical device manufacturer certified to the ISO 13485 standards.