描述
Hibernate Medium is a CO
2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium is optimized for embryonic cells or tissue in ambient carbon dioxide levels.
Hibernate Medium enables neurons to be maintained in ambient CO
2 levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.
Hibernate Medium may be combined with
B-27 Supplements to yield a complete medium.
For other applications, we provide alternative formulations of
Hibernate Medium and
B-27 Supplements.
For established protocols using Hibernate Medium, access our
Gibco Neurobiology Protocol Handbook.
View publications using Gibco neurobiology products at our
Gibco CellCite.
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