Anti-PHGDH Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PHGDH Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PHGDH magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PHGDH Magnetic Beads-IP Kit Product Description
The Anti-PHGDH magnetic Beads, conjugated with Anti-PHGDH antibody, are used for immuneprecipitation (IP) of PHGDH proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PHGDH proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PHGDH proteins are dissociated from the magnetic beads using an elution buffer. Anti-PHGDH Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PGDH / PHGDH Protein (Catalog#13167-H08E)
Species Reactivity
Human PGDH / PHGDH
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; Catalog#13167-H08E; O43175; Met1-Phe533). PGDH / PHGDH specific IgG was purified by Human PGDH / PHGDH affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PHGDH Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-3-PGDHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-3PGDHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-113ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NLSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NLS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PDGALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PGADALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PGDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PGDHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PHGDHDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SERAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PHGDH Background Information
PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.
Full Name
phosphoglycerate dehydrogenase
References
Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43. Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45. Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.
新浪微博
腾讯微博
QQ空间
客服
