Anti-GFP Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-GFP Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-GFP magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-GFP Magnetic Beads-IP Kit Product Description
The Anti-GFP magnetic Beads, conjugated with Anti-GFP antibody, are used for immuneprecipitation (IP) of GFP proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing GFP proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound GFP proteins are dissociated from the magnetic beads using an elution buffer. Anti-GFP Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
Species Reactivity
Aequorea victoria GFP
Source
Monoclonal Aequorea victoria Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser2-Lys238).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-GFP Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GFPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
GFP Background Information
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 58 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
References
Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637. Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248. Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067. Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028. Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161. Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
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