Anti-MAX Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-MAX Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-MAX magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-MAX Magnetic Beads-IP Kit Product Description
The Anti-MAX magnetic Beads, conjugated with Anti-MAX antibody, are used for immuneprecipitation (IP) of MAX proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MAX proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MAX proteins are dissociated from the magnetic beads using an elution buffer. Anti-MAX Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human MAX / MYC associated factor X protein (Catalog#12885-H20B)
Species Reactivity
Human MAX / MYC associated fac
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human MAX / MYC associated factor X (rh MAX / MYC associated factor X ; Catalog#12885-H20B; NP_002373; Met1-Ser160).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-MAX Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-bHLHd4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
MAX Background Information
MYC associated factor X contains 1 basic helix-loop-helix (bHLH) domain and belongs to MAX family. It is highly expressed in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. MYC associated factor X can form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. MYC associated factor X may also repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Multiple alternatively spliced transcript variants have been described for MYC associated factor X gene but the full-length nature for some of them is unknown.
Full Name
MYC associated factor X
References
Mac Partlin M, et al. (2003) Interactions of the DNA mismatch repair proteins MLH1 and MSH2 with c-MYC and MAX. Oncogene. 22(6):819-25. Cheng SW, et al. (1999) c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function. Nat enet. 22(1):102-5. McMahon SB, et al. (1998) The novel ATM-related protein TRRAP is an essential cofactor for the c-Myc and E2F oncoproteins. Cell. 94(3):363-74.
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