Anti-SOD2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SOD2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SOD2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SOD2 Magnetic Beads-IP Kit Product Description
The Anti-SOD2 magnetic Beads, conjugated with Anti-SOD2 antibody, are used for immuneprecipitation (IP) of SOD2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SOD2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SOD2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SOD2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SOD2 protein (Catalog#12656-HNAE)
Species Reactivity
Human SOD2
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SOD2 (rh SOD2; Catalog#12656-HNAE; P04179-1; Lys 25-Lys 222).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SOD2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-IPOBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MNSODALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MVCD6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SOD2 Background Information
Superoxide dismutases (SOD) are important anti-oxidant enzymes that guard against superoxide toxicity. In humans, as in all mammals and most chordates, three forms of superoxide dismutase (SOD) are present: SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. Mitochondrial superoxide dismutase [SOD; manganese SOD (MnSOD) or SOD2] neutralizes highly reactive superoxide radical (O(
Full Name
superoxide dismutase 2, mitochondrial
References
Culotta VC, et al. (2006) Activation of superoxide dismutases: putting the metal to the pedal. Biochim Biophys Acta. 1763(7): 747-58. Bag A, et al. (2008) Target sequence polymorphism of human manganese superoxide dismutase gene and its association with cancer risk: a review. Cancer Epidemiol Biomarkers Prev. 17(12): 3298-305. Diehl C, et al. (2009) The basis of topical superoxide dismutase antipruritic activity. Acta Dermatovenerol Croat. 17(1): 25-39. Ma X, et al. (2010) No association between SOD2 Val16Ala polymorphism and breast cancer susceptibility: a meta-analysis based on 9,710 cases and 11,041 controls. Breast Cancer Res Treat. 122(2): 509-14.
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