Anti-AK3L1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-AK3L1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-AK3L1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-AK3L1 Magnetic Beads-IP Kit Product Description
The Anti-AK3L1 magnetic Beads, conjugated with Anti-AK3L1 antibody, are used for immuneprecipitation (IP) of AK3L1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing AK3L1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound AK3L1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-AK3L1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human AK4 / AK3L1 protein (Catalog#12406-H20B)
Species Reactivity
Human AK4 / AK3L1
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human AK4 / AK3L1 (rh AK4 / AK3L1; Catalog#12406-H20B; P27144-1; Ala 2-Tyr 223).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-AK3L1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AK3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AK3L1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AK3L2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AK4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
AK3L1 Background Information
Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.
Full Name
adenylate kinase 4
References
Xu G., et al.,(1992), Characterization of human adenylate kinase 3 (AK3) cDNA and mapping of the AK3 pseudogene to an intron of the NF1 gene. Genomics 13:537-542. Gregory S.G., et al., (2006), The DNA sequence and biological annotation of human chromosome 1.Nature 441:315-321. Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.
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