Anti-CD44 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD44 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD44 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD44 Magnetic Beads-IP Kit Product Description
The Anti-CD44 magnetic Beads, conjugated with Anti-CD44 antibody, are used for immuneprecipitation (IP) of CD44 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD44 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD44 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD44 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CD44 protein (Catalog#12211-H08H)
Species Reactivity
Human CD44
Source
Monoclonal Human Mouse IgG1
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CD44 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CDW44ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CSPG8ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ECMR-IIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HCELLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HUTCH-IALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-INALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LHRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MC56ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MDU2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MDU3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIC4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Pgp1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CD44 Background Information
CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.
Full Name
CD44 molecule (Indian blood group)
References
Bajorath J. (2000) Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions. Proteins. 39(2): 103-11. Johnson P, et al. (2000) A role for the cell adhesion molecule CD44 and sulfation in leukocyte-endothelial cell adhesion during an inflammatory response? Biochem Pharmacol. 59(5): 455-65. Martin TA, et al. (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol. 46(2): 165-86. Toole BP, et al. (2008) Hyaluronan, CD44 and Emmprin: partners in cancer cell chemoresistance. Drug Resist Updat. 11(3): 110-21. Johnson P, et al. (2009) CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 8(3): 208-20.
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