Anti-Alpha-fetoprotein Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Alpha-fetoprotein Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Alpha-fetoprotein magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Alpha-fetoprotein Magnetic Beads-IP Kit Product Description
The Anti-Alpha-fetoprotein magnetic Beads, conjugated with Anti-Alpha-fetoprotein antibody, are used for immuneprecipitation (IP) of Alpha-fetoprotein proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Alpha-fetoprotein proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Alpha-fetoprotein proteins are dissociated from the magnetic beads using an elution buffer. Anti-Alpha-fetoprotein Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human AFP / alpha-fetoprotein protein (Catalog#12177-H08H)
Species Reactivity
Human AFP / alpha-fetoprotein
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; Catalog#12177-H08H; P02771; Met1-Val609).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Alpha-fetoprotein Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AFPDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Alpha-fetoproteinALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FETAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HPAFPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Alpha-fetoprotein Background Information
Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
alpha-fetoprotein
References
Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408. Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65. Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
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