Anti-GSTM2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-GSTM2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-GSTM2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-GSTM2 Magnetic Beads-IP Kit Product Description
The Anti-GSTM2 magnetic Beads, conjugated with Anti-GSTM2 antibody, are used for immuneprecipitation (IP) of GSTM2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing GSTM2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound GSTM2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-GSTM2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human GSTM2 protein (Catalog#12042-H07E)
Species Reactivity
Human GSTM2
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human GSTM2 (rh GSTM2; Catalog#12042-H07E; P28161; Met1-Lys218).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-GSTM2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GST4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GSTMALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GSTM2-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GTHMUSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
GSTM2 Background Information
Glutathione S-transferase Mu 2, also known as GST class-mu 2, GSTM2-2 and GSTM2, is a cytoplasm protein which belongs to the GST superfamily and Mu family. GSTM2 / GST4 contains one GST C-terminal domain and one GST N-terminal domain. The glutathione S-transferases (GSTs) are a multigene family of enzymes largely involved in the detoxification of chemicals. Eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. Butyrate, an important luminal component produced from fermentation of dietary fibers, is an efficient inducer of GSTs and especially of GSTM2. Butyrate may act chemoprotectively by increasing detoxification capabilities in the colon mucosa.
Full Name
glutathione S-transferase mu 2 (muscle)
References
Campbell E, et al.,1990, J Biol Chem 265 (16): 9188-93. Vorachek WR, et al.,1991, Proc Natl Acad Sci USA. 88 (10): 4443-7. Ebert,M.N. et al., 2003, Carcinogenesis. 24 (10):1637-44.
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