Anti-Tryptophan Hydroxylase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Tryptophan Hydroxylase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Tryptophan Hydroxylase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Tryptophan Hydroxylase Magnetic Beads-IP Kit Product Description
The Anti-Tryptophan Hydroxylase magnetic Beads, conjugated with Anti-Tryptophan Hydroxylase antibody, are used for immuneprecipitation (IP) of Tryptophan Hydroxylase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Tryptophan Hydroxylase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Tryptophan Hydroxylase proteins are dissociated from the magnetic beads using an elution buffer. Anti-Tryptophan Hydroxylase Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Tryptophan Hydroxylase 1 / TPH1 Protein (Catalog#11931-H07E)
Species Reactivity
Human Tryptophan Hydroxylase 1
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Tryptophan Hydroxylase 1 / TPH1 (rh Tryptophan Hydroxylase 1 / TPH1; Catalog#11931-H07E; P17752-1; Ile2-Ile444).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Tryptophan Hydroxylase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-TPRHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TRPHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Tryptophan Hydroxylase Background Information
Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme which belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells.The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior.
Full Name
tryptophan hydroxylase 1
References
Antonia S. New, et al.,1998, American Journal of Medical Genetics Part B. 81 (1): 13-17. Y. C Wang, et al.,2001, Neuropsychobiology. 43 (1): 1 - 4. Diego J. et al., 2003, Science. 299 (5603): 76. Allen NC, et al.,2008, Nat Genet. 40 (7): 827-34. Galfalvy,H. et al., 2009, J Affect Disord. 115 (3):331-8.
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