Anti-C1QBP Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-C1QBP Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-C1QBP magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-C1QBP Magnetic Beads-IP Kit Product Description
The Anti-C1QBP magnetic Beads, conjugated with Anti-C1QBP antibody, are used for immuneprecipitation (IP) of C1QBP proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing C1QBP proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound C1QBP proteins are dissociated from the magnetic beads using an elution buffer. Anti-C1QBP Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human C1QBP / HABP1 protein (Catalog#11874-H08E)
Species Reactivity
Human C1QBP / HABP1
Source
Monoclonal Human Mouse IgG2b
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human C1QBP / HABP1 (rh C1QBP / HABP1; Catalog#11874-H08E; NP_001203.1; His 75-Gln 282). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-C1QBP Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-gC1Q-RALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GC1QBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-gC1qRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HABP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p32ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SF2p32ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
C1QBP Background Information
Hyaluronan binding protein 1 (HABP1), also known as p32 or gC1qR, is a ubiquitously expressed multifunctional phospho-protein implicated in cell signalling. Hyaluronan-binding protein 1 (HABP1) /p32/gC1qR was characterized as a highly acidic and oligomeric protein, which binds to different ligands like hyaluronan, C1q, and mannosylated albumin. The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro. HABP1 overexpressing cells showed extensive vacuolation and reduced growth rate, which was corrected by frequent medium replenishment. Further investigation revealed that HABP1 overexpressing cells undergo apoptosis, and they failed to enter into the S-phase. The sperm surface HABP1 level can be correlated with the degree of sperm motility.Hyaluronan binding protein 1 (HABP1) was reported to be present on human sperm surface and its involvement in fertilization has already been elucidated: decreased HABP1 level may be associated with low motility of sperms, which in turn might cause infertility in the patient. HABP1 also is an endogenous substrate for MAP kinase and upon mitogenic stimulation it is translocated to the nucleus in a MAP kinase-dependent manner.
Full Name
complement component 1, q subcomponent binding protein
References
Meenakshi J, et al. (2003) Constitutive expression of hyaluronan binding protein 1 (HABP1/p32/gC1qR) in normal fibroblast cells perturbs its growth characteristics and induces apoptosis. Biochemicaland Biophysical Research Communication. 300(3): 686-93. Majumdar M, et al. (2002) Hyaluronan Binding Protein 1 (HABP1) /C1QBP/p32 Is an Endogenous Substrate for MAP Kinase and Is Translocated to the Nucleus upon Mitogenic Stimulation. Biochemical and Biophysical Research Communications. 291(4): 829-37. Ghosh I, et al. (2002) Reduction in the level of hyaluronan binding protein 1 (HABP1) is associated with loss of sperm motility. Journal of Reproductive Immunology. 53(1-2): 45-54.
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