Anti-HIV gp140 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-HIV gp140 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-HIV gp140 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-HIV gp140 Magnetic Beads-IP Kit Product Description
The Anti-HIV gp140 magnetic Beads, conjugated with Anti-HIV gp140 antibody, are used for immuneprecipitation (IP) of HIV gp140 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing HIV gp140 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound HIV gp140 proteins are dissociated from the magnetic beads using an elution buffer. Anti-HIV gp140 Magnetic Beads-IP Kit Antibody Information
Antibody
HIV-1 gp140 (group M, subtype CRF07_BC) Antibody, Rabbit PAb, Antigen Affinity Purified(
11677-T52)
Immunogen
Recombinant HIV-1 gp140 (group M, subtype CRF07_BC) Protein (Catalog#11677-V08H)
Species Reactivity
HIV-1 gp140 (group M, subtype
Source
Polyclonal HIV Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant HIV-1 gp140 (group M, subtype CRF07_BC) ( Catalog#11677-V08H; ; Val30-Tyr677). HIV-1 gp140 (group M, subtype CRF07_BC) specific IgG was purified by HIV-1 gp140 (group M, subtype CRF07_BC) affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
HIV gp140 Background Information
Full Name
CUB domain containing protein 1
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