Anti-Hemagglutinin / HA Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Hemagglutinin / HA Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Hemagglutinin / HA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Hemagglutinin / HA Magnetic Beads-IP Kit Product Description
The Anti-Hemagglutinin / HA magnetic Beads, conjugated with Anti-Hemagglutinin / HA antibody, are used for immuneprecipitation (IP) of Hemagglutinin / HA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Hemagglutinin / HA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Hemagglutinin / HA proteins are dissociated from the magnetic beads using an elution buffer. Anti-Hemagglutinin / HA Magnetic Beads-IP Kit Antibody Information
Antibody
Influenza H1N1 (A/California/07/2009) Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified(
11085-T54)
Immunogen
Recombinant Influenza H1N1 (A/California/07/2009) Hemagglutinin / HA Protein (Catalog#11085-V08H)
Species Reactivity
Influenza H1N1 (A/California/0
Source
Polyclonal H1N1 Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Influenza H1N1 (A/California/07/2009) Hemagglutinin / HA ( Catalog#11085-V08H; ACP44189.1; Met1-Gln529). Influenza H1N1 (A/California/07/2009) Hemagglutinin / HA specific IgG was purified by Influenza H1N1 (A/California/07/2009) Hemagglutinin / HA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Hemagglutinin / HA Background Information
The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses. Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408. Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
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