Mouse FABP4 HEK293 Overexpression Lysate 产品信息
Product Description
This Mouse FABP4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FABP4 protein (Cat: 50652-M35H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Sequence Information
A DNA sequence encoding the mouse Fabp4 (NP_077717.1) (Cys2-Ala132) was expressed with a polyhistidine tag at the N-terminus and a myc tag at the C-terminus.
Molecule Mass
The recombinant mouse Fabp4 consists of 147 amino acids and predicts a molecular mass of 16.5 kDa.
Mouse FABP4 HEK293 Overexpression Lysate Usage Guide
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Mouse FABP4 HEK293 Overexpression Lysate Alternative Names
Mouse 422/aP2 Overexpression Lysate;Mouse ALBP/Ap2 Overexpression Lysate;Mouse Ap2 Overexpression Lysate;Mouse Lbpl Overexpression Lysate
FABP4 Background Information
Fatty acid-binding protein, adipocyte, also known as Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, and FABP4, is a cytoplasm protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. In familial combined hyperlipidemia (FCHL), FABP4 correlated to body mass index (BMI), waist circumference and homeostasis model assessment (HOMA) index.FABP4 levels were associated with triglyceride-rich lipoproteins. In humans serum FABP4 levels correlate significantly with features of PCOS. It appears to be a determinant of atherogenic dyslipidemia. FABP4 pathway mediates the sebaceous gland hyperplasia in keratinocyte-specific Pten-null mice. FABP4 concentration significantly increased with an increasing of MS features and was strongly correlated with body-mass index, triglycerides, HDL-cholesterol concentrations and blood pressure. Patients in the highest quartile of FABP4 presented a six-fold increased odds ratio for MS and a three-fold increased odds for LD, adjusted by age, sex, body-mass index and the antiretroviral therapy. FABP4 is a strong plasma marker of metabolic disturbances in HIV-infected patients, and therefore, could serve to guide therapeutic intervention in this group of patients.
Full Name
fatty acid binding protein 4, adipocyte
References
van Dongen,M.J. et al., 2002, J Am Chem Soc. 124 (40): 11874-80. Coll, B. et al., 2008, Atherosclerosis 199 (1):147-53. Hoashi,S. et al., 2008, BMC Genet. 9 :84. Cai,H. et al., 2010, Bioorg Med Chem Lett 20 (12):3675-9.
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