Anti-RBP4 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-RBP4 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-RBP4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-RBP4 Magnetic Beads-IP Kit Product Description
The Anti-RBP4 magnetic Beads, conjugated with Anti-RBP4 antibody, are used for immuneprecipitation (IP) of RBP4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing RBP4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound RBP4 proteins are dissociated from the magnetic beads using an elution buffer. Anti-RBP4 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Cynomolgus RBP4 protein (Catalog#90220-C08H)
Species Reactivity
Cynomolgus RBP4
Source
Polyclonal Cynomolgus Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus RBP4 (rh RBP4; Catalog#90220-C08H; 0; Met1-Leu201). RBP4 specific IgG was purified by Cynomolgus RBP4 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
RBP4 Background Information
Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).
Full Name
retinol binding protein 4, plasma
References
Yang Q, et al. (2005) Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 436(7049): 356-62. Choi SH, et al. (2008) High plasma retinol binding protein-4 and low plasma adiponectin concentrations are associated with severity of glucose intolerance in women with previous gestational diabetes mellitus. J Clin Endocrinol Metab. 93(8): 3142-8. Tepper BJ, et al. (2010) Serum retinol-binding protein 4 (RBP4) and retinol in a cohort of borderline obese women with and without gestational diabetes. Clin Biochem. 43(3): 320-3.
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