Anti-IL-18 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-IL-18 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-IL-18 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-IL-18 Magnetic Beads-IP Kit Product Description
The Anti-IL-18 magnetic Beads, conjugated with Anti-IL-18 antibody, are used for immuneprecipitation (IP) of IL-18 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IL-18 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IL-18 proteins are dissociated from the magnetic beads using an elution buffer. Anti-IL-18 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Rhesus IL18 / Interleukin-18 protein (Catalog#90011-C07E)
Species Reactivity
Cynomolgus IL18 / Interleukin-
Source
Polyclonal Rhesus Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus IL18 / Interleukin-18 (rC IL18 / Interleukin-18; Catalog#90011-C07E; Ala 2-Asp 193). IL18 / Interleukin-18 specific IgG was purified by Cynomolgus IL18 / Interleukin-18 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
IL-18 Background Information
Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1?like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4? and STAT6?dependent fashion. IL-18 and T cell receptor?mediated stimulation could induce naïve CD4+ T cells to develop into IL-4?producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy References
Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24. Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90. Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.