Anti-EphB3 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-EphB3 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-EphB3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-EphB3 Magnetic Beads-IP Kit Product Description
The Anti-EphB3 magnetic Beads, conjugated with Anti-EphB3 antibody, are used for immuneprecipitation (IP) of EphB3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing EphB3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound EphB3 proteins are dissociated from the magnetic beads using an elution buffer. Anti-EphB3 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Rat EphB3 / HEK2 / Eph Receptor B3 Protein (Catalog#81593-R08H)
Species Reactivity
Rat EphB3 / HEK2 / Eph Recepto
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat EphB3 / HEK2 / Eph Receptor B3 (rR EphB3 / HEK2 / Eph Receptor B3; Catalog#81593-R08H; NP_001099338.1; Met1-Thr537). EphB3 / HEK2 / Eph Receptor B3 specific IgG was purified by Rat EphB3 / HEK2 / Eph Receptor B3 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
EphB3 Background Information
Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA1, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
Full Name
EPH receptor B3
References
Bergemann AD, et al. (1998) Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 16(4): 471-80. Hock B, et al. (1998) PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor. Proc Natl Acad Sci U S A. 95(17): 9779-84. Liu X, et al. (2006) EphB3: an endogenous mediator of adult axonal plasticity and regrowth after CNS injury. J Neurosci. 26(12): 3087-101.
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