Anti-ENO1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ENO1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ENO1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ENO1 Magnetic Beads-IP Kit Product Description
The Anti-ENO1 magnetic Beads, conjugated with Anti-ENO1 antibody, are used for immuneprecipitation (IP) of ENO1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ENO1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ENO1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ENO1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Rat ENO1 / Enolase 1 / alpha-enolase protein (Catalog#80578-R07E)
Species Reactivity
Rat ENO1 / Enolase 1 / alpha-e
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat ENO1 / Enolase 1 / alpha-enolase (rh ENO1 / Enolase 1 / alpha-enolase; Catalog#80578-R07E; NP_036686.2; Met1-Lys434). ENO1 / Enolase 1 / alpha-enolase specific IgG was purified by Rat ENO1 / Enolase 1 / alpha-enolase affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ENO1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Eno-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ENO1 Background Information
The ENO1 gene encodes a multifunctional enzyme that has been identified as a key component of the glycolytic pathway. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. The results of the ENO1 expression profiling of ovarian follicles suggest that ENO1 may play an important dual role in the progress of follicular development, where ENO1 acts as a glycolytic enzyme and also mediates apoptosis. ENO1 overexpression could make the primary culture follicle granulosa cells in vitro improve progesterone secretion. The over-expression of ENO1 protein can enhance the abilities of proliferation and migration in gastric cancer cells of AGS, which indicates that ENO1 may be an important potential tumor-marker associated with the development of gastric cancer. ENO1 and GPI can be used as markers of human sperm freezability before starting the cryopreservation procedure. The inhibition of ENO1 expression may be a novel strategy for therapy for NHLs patients, and it may be a target for drug resistance.
Full Name
enolase 1, (alpha)
References
Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74. Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7. Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6. Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.
新浪微博
腾讯微博
QQ空间
客服
