Anti-B7-H3 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-B7-H3 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-B7-H3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-B7-H3 Magnetic Beads-IP Kit Product Description
The Anti-B7-H3 magnetic Beads, conjugated with Anti-B7-H3 antibody, are used for immuneprecipitation (IP) of B7-H3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing B7-H3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound B7-H3 proteins are dissociated from the magnetic beads using an elution buffer. Anti-B7-H3 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Rat B7-H3 / CD276 protein (Catalog#80380-R08H)
Species Reactivity
Rat B7-H3 / CD276
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#80380-R08H; Q7TPB4; Met1-Phe244). B7-H3 / CD276 specific IgG was purified by Rat B7-H3 / CD276 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
B7-H3 Background Information
References
Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73. Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8. Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875. Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12. Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.