Anti-CD48 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD48 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD48 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD48 Magnetic Beads-IP Kit Product Description
The Anti-CD48 magnetic Beads, conjugated with Anti-CD48 antibody, are used for immuneprecipitation (IP) of CD48 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD48 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD48 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD48 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Rat CD48 / SLAMF2 protein (Catalog#80286-R08H)
Species Reactivity
Rat CD48 / SLAMF2
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat CD48 / SLAMF2 (rh CD48 / SLAMF2; Catalog#80286-R08H; P10252; Met1-Arg216). CD48 / SLAMF2 specific IgG was purified by Rat CD48 / SLAMF2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
CD48 Background Information
References
Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50 Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
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