Anti-Her2 / ERBB2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Her2 / ERBB2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Her2 / ERBB2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Her2 / ERBB2 Magnetic Beads-IP Kit Product Description
The Anti-Her2 / ERBB2 magnetic Beads, conjugated with Anti-Her2 / ERBB2 antibody, are used for immuneprecipitation (IP) of Her2 / ERBB2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Her2 / ERBB2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Her2 / ERBB2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Her2 / ERBB2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Rat HER2 / ErbB2 Protein (Catalog#80079-R08H)
Species Reactivity
Rat HER2 / ErbB2
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat HER2 / ErbB2 (rR HER2 / ErbB2; Catalog#80079-R08H; AAH61863.1; Met4-Thr656). HER2 / ErbB2 specific IgG was purified by Rat HER2 / ErbB2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Her2 / ERBB2 Background Information
Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
erb-b2 receptor tyrosine kinase 2
References
Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
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