Anti-CD16a Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CD16a Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CD16a magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CD16a Magnetic Beads-IP Kit Product Description
The Anti-CD16a magnetic Beads, conjugated with Anti-CD16a antibody, are used for immuneprecipitation (IP) of CD16a proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD16a proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD16a proteins are dissociated from the magnetic beads using an elution buffer. Anti-CD16a Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Rat CD16a / FCGR3A protein (Catalog#80017-R08H)
Species Reactivity
Rat CD16a / FCGR3A
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat CD16a / FCGR3A (rR CD16a / FCGR3A; Catalog#80017-R08H; Met 1-Pro 200; ABO70343.1). CD16a / FCGR3A specific IgG was purified by Rat CD16a / FCGR3A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
CD16a Background Information
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Full Name
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
References
David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
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