Anti-Carbonic Anhydrase IX Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Carbonic Anhydrase IX Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Carbonic Anhydrase IX magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Carbonic Anhydrase IX Magnetic Beads-IP Kit Product Description
The Anti-Carbonic Anhydrase IX magnetic Beads, conjugated with Anti-Carbonic Anhydrase IX antibody, are used for immuneprecipitation (IP) of Carbonic Anhydrase IX proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Carbonic Anhydrase IX proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Carbonic Anhydrase IX proteins are dissociated from the magnetic beads using an elution buffer. Anti-Carbonic Anhydrase IX Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Carbonic Anhydrase IX Antibody(
70028-T52)
Immunogen
Recombinant Canine Carbonic Anhydrase IX / CA9 protein (Catalog#70028-D08H)
Species Reactivity
Canine Carbonic Anhydrase IX /
Source
Polyclonal Canine Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Canine Carbonic Anhydrase IX / CA9 (rh Carbonic Anhydrase IX / CA9; Catalog#70028-D08H; NP_001138646.1; Met1-Leu410). Carbonic Anhydrase IX / CA9 specific IgG was purified by Canine Carbonic Anhydrase IX / CA9 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Carbonic Anhydrase IX Background Information
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy Full Name
carbonic anhydrase IX
References
Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9. Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71. Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27. Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.
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