Anti-LDHA Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-LDHA Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-LDHA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-LDHA Magnetic Beads-IP Kit Product Description
The Anti-LDHA magnetic Beads, conjugated with Anti-LDHA antibody, are used for immuneprecipitation (IP) of LDHA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing LDHA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound LDHA proteins are dissociated from the magnetic beads using an elution buffer. Anti-LDHA Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse LDH-A / LDHA Protein (Catalog#51207-M07E)
Species Reactivity
Mouse LDH-A / LDHA
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse LDH-A / LDHA (rM LDH-A / LDHA; Catalog#51207-M07E; NP_034829.1; Met1-Phe332). LDH-A / LDHA specific IgG was purified by Mouse LDH-A / LDHA affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-LDHA Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-l7R2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ldh1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LdhmALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
LDHA Background Information
Lactate dehydrogenase A (LDHA), a key enzyme regulating aerobic glycolysis, is overexpressed in many human cancers, and correlates with poor clinical outcomes.Lactate dehydrogenase-A (LDHA) can promote Warburg effect to produce lactate and Adenosine Triphosphate (ATP) in aerobic condition, which contributes to oncogenesis metastasis and drug resistance in various cancers.Up-regulation of lactate dehydrogenase LDHA, is a frequent event in human malignancies and relate to poor postoperative outcome.
Full Name
lactate dehydrogenase A