Anti-IL36 gamma Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-IL36 gamma Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-IL36 gamma magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-IL36 gamma Magnetic Beads-IP Kit Product Description
The Anti-IL36 gamma magnetic Beads, conjugated with Anti-IL36 gamma antibody, are used for immuneprecipitation (IP) of IL36 gamma proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IL36 gamma proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IL36 gamma proteins are dissociated from the magnetic beads using an elution buffer. Anti-IL36 gamma Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse IL36G protein (Catalog#51179-M07E)
Species Reactivity
Mouse IL36G
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse IL36G (rh IL36G; Catalog#51179-M07E; Q8R460; Gly13-Ser164). IL36G specific IgG was purified by Mouse IL36G affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-IL36 gamma Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Il1f9ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IL36gALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
IL36 gamma Background Information
Full Name
interleukin 36, gamma
References
Dinarello CA. (2002) The IL-1 family and inflammatory diseases. Clin Exp Rheumatol. 20(5): 1-13. Berglof E, et al. (2003) IL-1Rrp2 expression and IL-1F9 (IL-1H1) actions in brain cells. J Neuroimmunol. 139(1-2): 36-43. Dunn E, et al. (2001) Annotating genes with potential roles in the immune system: six new members of the IL-1 family. Trends Immunol.22(10): 533-6. Towne JE, et al. (2004) Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J Biol Chem. 279(14): 13677-88.