Anti-H Cadherin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-H Cadherin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-H Cadherin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-H Cadherin Magnetic Beads-IP Kit Product Description
The Anti-H Cadherin magnetic Beads, conjugated with Anti-H Cadherin antibody, are used for immuneprecipitation (IP) of H Cadherin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing H Cadherin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound H Cadherin proteins are dissociated from the magnetic beads using an elution buffer. Anti-H Cadherin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CDH13 / Cadherin-13 / H Cadherin protein (Catalog#51163-M08H)
Species Reactivity
Mouse CDH13 / Cadherin-13 / H
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CDH13 / Cadherin-13 / H Cadherin (rh CDH13 / Cadherin-13 / H Cadherin; Catalog#51163-M08H; NP_062681.2; Met1-Ala692). CDH13 / Cadherin-13 / H Cadherin specific IgG was purified by Mouse CDH13 / Cadherin-13 / H Cadherin affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-H Cadherin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-4932416G01RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CdhtALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-T-cadherinALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TcadALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
H Cadherin Background Information
CDH13, also known as cadherin-13 and H Cadherin, is a member of the cadherin superfamily. CDH13 acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. CDH13 is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. CDH13 gene is hypermethylated in many types of cancer.
References
Hart AB. et al., 2012, PLoS One. 7 (8): e42646. Xu J. et al., 2012, BMC Cancer. 12: 243. Jo J. et al., 2012, Obesity (Silver Spring). 20 (8): 1683-7.
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