Anti-Aconitase 2 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Aconitase 2 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Aconitase 2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Aconitase 2 Magnetic Beads-IP Kit Product Description
The Anti-Aconitase 2 magnetic Beads, conjugated with Anti-Aconitase 2 antibody, are used for immuneprecipitation (IP) of Aconitase 2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Aconitase 2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Aconitase 2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Aconitase 2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse ACO2 / Aconitase 2 protein (Catalog#51015-M20B)
Species Reactivity
Mouse ACO2 / Aconitase 2
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse ACO2 / Aconitase 2 (rh ACO2 / Aconitase 2; Catalog#51015-M20B; Q99KI0; Gln28-Gln780). ACO2 / Aconitase 2 specific IgG was purified by Mouse ACO2 / Aconitase 2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Aconitase 2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Aco-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Aco3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-D10Wsu183eALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Aconitase 2 Background Information
A homozygous missense mutation was identified in the ACO2 gene (c.124T>G p.Phe414Val) that segregated with HSP complicated by intellectual disability and microcephaly. Lymphoblastoid cell lines of homozygous carrier patients revealed significantly decreased activity of the mitochondrial aconitase enzyme and defective mitochondrial respiration. ACO2 encodes mitochondrial aconitase, an essential enzyme in the Krebs cycle. Recessive mutations in this gene have been previously associated with cerebellar ataxia. We found homozygous or compound heterozygous missense and frameshift mutations in the gene encoding mitochondrial aconitase (ACO2), a tricarboxylic acid cycle enzyme, catalysing interconversion of citrate into isocitrate. Unlike wild type ACO2, all mutant ACO2 proteins failed to complement the respiratory growth of a yeast aco1-deletion strain. The study shows that autosomal recessive ACO2 mutations can cause either isolated or syndromic optic neuropathy. This observation identifies ACO2 as the second gene responsible for non-syndromic autosomal recessive optic neuropathies and provides evidence for a genetic overlap between isolated and syndromic forms, giving further support to the view that optic atrophy is a hallmark of defective mitochondrial energy supply.
Full Name
aconitase 2, mitochondrial
References
Robbins AH, et al. (1989) The structure of aconitase. Proteins. 5 (4): 289-312. Lauble H, et al. (1992) Crystal structures of aconitase with isocitrate and nitroisocitrate bound. Biochemistry. 31 (10): 2735-48. Robbins AH, et al. (1989) Structure of activated aconitase: formation of the 4Fe-4S cluster in the crystal. Proc Natl Acad Sci. 86 (10): 3639-43.