Anti-FCRL1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-FCRL1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-FCRL1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-FCRL1 Magnetic Beads-IP Kit Product Description
The Anti-FCRL1 magnetic Beads, conjugated with Anti-FCRL1 antibody, are used for immuneprecipitation (IP) of FCRL1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing FCRL1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound FCRL1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-FCRL1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse FCRL1 / FCRH1 protein (Catalog#50947-M08H)
Species Reactivity
Mouse FCRL1 / FCRH1
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse FCRL1 / FCRH1 (rh FCRL1 / FCRH1; Catalog#50947-M08H; Q8R4Y0-2; Met1-Thr204). FCRL1 / FCRH1 specific IgG was purified by Mouse FCRL1 / FCRH1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-FCRL1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-A230020G22RikALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Bxmas1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Bxmh1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Bxmh1bALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Fcrh1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Fcrh1lALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Fcrh1sALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ifgp1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
FCRL1 Background Information
Fc receptor-like protein 1, also known as FcR-like protein 1, Fc receptor homolog 1, IFGP family protein 1, Immune receptor translocation-associated protein 5 and FCRL1, is a single-pass type I membrane protein which contains three Ig-like C2-type (immunoglobulin-like) domains. It is a cell-surface membrane protein belonging to FCRL family and is preferentially expressed on B cells. FCRL1 is primarily expressed in secondary lymphoid tissues by mature subsets of B cells. It is detected in spleen, lymph node, heart, skeletal muscle, kidney, liver and placenta. FCRL1 is specifically expressed by mature B lineage cells with higher expression in naive versus memory B cells (at protein level). Human Fc receptor-like molecules (FCRL1, FCRL2, FCRL3, FCRL4, FCRL5) have tyrosine-based immunoregulatory potential and are expressed by B-lineage subpopulations. FCRL1 may function as an activating coreceptor in B cells. It may also function in B cells activation and differentiation.
Full Name
Fc receptor-like 1
References
Du X. et al., 2008, Blood. 111 (1): 338-43. Kazemi T. et al., 2008, Int J Cancer. 123 (9): 2113-9. Baranov K. et al., 2008, J Immunol Methods. 332 (1-2): 73-81.
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