Anti-SHP-1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SHP-1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SHP-1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SHP-1 Magnetic Beads-IP Kit Product Description
The Anti-SHP-1 magnetic Beads, conjugated with Anti-SHP-1 antibody, are used for immuneprecipitation (IP) of SHP-1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SHP-1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SHP-1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SHP-1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse PTPN6 Protein (Catalog#50894-M20B)
Species Reactivity
Mouse PTPN6
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse PTPN6 (rM PTPN6; Catalog#50894-M20B; P29351-2; Ala207-Lys597). PTPN6 specific IgG was purified by Mouse PTPN6 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SHP-1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-70Z-SHPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-hcpALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HcphALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-meALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Ptp1CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PTPTY-42ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SH-PTP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SHP-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SHP-1 Background Information
PTPN6 is an enzyme which belongs to the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of PTPN6 contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of PTPN6 with its substrates. PTPN6 is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. It has been shown that PTPN6 interacts with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.
Full Name
protein tyrosine phosphatase, non-receptor type 6
References
Yu CL, et al. (2000) Cytosolic tyrosine dephosphorylation of STAT5. Potential role of SHP-2 in STAT5 regulation. J Biol Chem. 275(1):599-604. Wu DW, et al. (2000) SH2-Containing protein tyrosine phosphatase-1 (SHP-1) association with Jak2 in UT-7/Epo cells. Blood Cells Mol Dis. 26(1):15-24. Jiao H, et al. (1996) Direct association with and dephosphorylation of Jak2 kinase by the SH2-domain-containing protein tyrosine phosphatase SHP-1. Mol Cell Biol. 16(12):6985-92.
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