Anti-Thimet Oligopeptidase Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Thimet Oligopeptidase Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Thimet Oligopeptidase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Thimet Oligopeptidase Magnetic Beads-IP Kit Product Description
The Anti-Thimet Oligopeptidase magnetic Beads, conjugated with Anti-Thimet Oligopeptidase antibody, are used for immuneprecipitation (IP) of Thimet Oligopeptidase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Thimet Oligopeptidase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Thimet Oligopeptidase proteins are dissociated from the magnetic beads using an elution buffer. Anti-Thimet Oligopeptidase Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse THOP1 protein (Catalog#50828-M07B)
Species Reactivity
Mouse THOP1
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse THOP1 (rM THOP1; Catalog#50828-M07B; NP_073144.3; Lys 2-Cys687). THOP1 specific IgG was purified by Mouse THOP1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Thimet Oligopeptidase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AI131655ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-AI327041ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EP24.15ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Thimet Oligopeptidase Background Information
THOP1, also known as Thimet oligopeptidase 1, Thimet oligopeptidase, EC 3.4.24.15, or EP24.15, is a zinc(II) endopeptidase implicated in the processing of numerous physiological peptides. As an intracellular enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue, THOP1 has been proposed to metabolize peptides within cells, thereby affecting antigen presentation and G protein-coupled receptor signal transduction. Its substrates is gonadotrophin-releasing hormone (GnRH), an important hypothalamic hormone that regulates the synthesis and release of oestradiol and facilitates female sexual behaviour. THOP1 against toxic effects of Abeta in the early stages of Alzheimer disease (AD) pathology, and suggest that the observed increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load.
Full Name
thimet oligopeptidase 1
References
Cyr NE, et al. (2010) Nuclear Thimet oligopeptidase is coexpressed with oestrogen receptor alpha in hypothalamic cells and regulated by oestradiol in female mice. J Neuroendocrinol. 22(8): 936-43. Berti DA, et al. (2009) Analysis of intracellular substrates and products of thimet oligopeptidase in human embryonic kidney 293 cells. J Biol Chem. 284(21): 14105-16. Russo LC, et al. (2009) Interaction with calmodulin is important for the secretion of thimet oligopeptidase following stimulation. FEBS J. 276(16): 4358-71. Pollio G, et al. (2008) Increased expression of the oligopeptidase THOP1 is a neuroprotective response to Abeta toxicity. Neurobiol Dis. 31(1): 145-58. Bruce LA, et al. (2008) Hydrogen bond residue positioning in the 599-611 loop of thimet oligopeptidase is required for substrate selection. FEBS J. 275(22): 5607-17.