Anti-CDC37 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CDC37 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CDC37 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CDC37 Magnetic Beads-IP Kit Product Description
The Anti-CDC37 magnetic Beads, conjugated with Anti-CDC37 antibody, are used for immuneprecipitation (IP) of CDC37 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CDC37 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CDC37 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CDC37 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CDC37 protein (Catalog#50826-M20B)
Species Reactivity
Mouse CDC37
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CDC37 (rh CDC37; Catalog#50826-M20B; Q61081; Met1-Ala379). CDC37 specific IgG was purified by Mouse CDC37 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CDC37 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-p50ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p50Cdc37ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CDC37 Background Information
CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is though to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp9. Decreased Hsp9 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified to Hsp9's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.
Full Name
cell division cycle 37
References
Dai K, et al. (1996) P hysical Interaction of Mammalian CDC37 with CDK4. The journal of biological chemistry. 271: 22030-4. Pearl LH, et al. (2005) Hsp90 and Cdc37-a chaperone cancer conspiracy. Current opinion in genetics development. 15 (1): 55-61. Chen GQ, et al. (2002) TNF-Induced Recruitment and Activation of the IKK Complex Require Cdc37 and Hsp90. Molecular cell. 9 (2): 401-10.
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