Anti-CK2 alpha Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-CK2 alpha Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CK2 alpha magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CK2 alpha Magnetic Beads-IP Kit Product Description
The Anti-CK2 alpha magnetic Beads, conjugated with Anti-CK2 alpha antibody, are used for immuneprecipitation (IP) of CK2 alpha proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CK2 alpha proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CK2 alpha proteins are dissociated from the magnetic beads using an elution buffer. Anti-CK2 alpha Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Mouse CSNK2A1 / CK2A1 protein (Catalog#50818-M20B)
Species Reactivity
Mouse CSNK2A1 / CK2A1
Source
Polyclonal Mouse Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CSNK2A1 / CK2A1 (rM CSNK2A1 / CK2A1; Catalog#50818-M20B; Q60737; Met1-Gln391). CSNK2A1 / CK2A1 specific IgG was purified by Mouse CSNK2A1 / CK2A1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CK2 alpha Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-Csnk2a1-rs4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CK2 alpha Background Information
Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp9, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via th phosphorylation of Akt.
Full Name
casein kinase 2, alpha 1 polypeptide
References
Schlpfer J, et al. (1997) A radiation hybrid framework map of bovine chromosome 13. Chromosome Res. 5(8): 511-9. Wirkner U, et al. (1994) The human gene (CSNK2A1) coding for the casein kinase II subunit alpha is located on chromosome 20 and contains tandemly arranged Alu repeats. Genomics. 19(2): 257-65. Wirkner U, et al. (1998) Genomic organization and promoter identification of the human protein kinase CK2 catalytic subunit alpha (CSNK2A1). Genomics. 48(1): 71-8.